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ecori sites
Ecori Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori+site/EcoR+I/pm42092416-72-36-42
Average 99 stars, based on 15959 article reviews
Ecori Sites, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 15959 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori+site/EcoR+I/pm42092416-72-36-42
Average 99 stars, based on 15959 article reviews
ecori sites - by Bioz Stars,
2026-09
99/100 stars
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Clone Assay:Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D. Article Snippet: .. The various FD cDNAs were cloned into the pCAGGS vector 21 at the Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 1-181 and 1030-1218 of A. thaliana SMC1 fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Article Title: Transcriptional adapter ADA2 regulates yield and end-use quality through liquid-liquid phase separation in wheat. Article Snippet: .. The full‐length GCN5, along with the introduced mutation and some domain deletions, was cloned into the Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 2-169 and 1056-1217 of M. musculus SMC1β fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D Article Snippet: Human and mouse pro- FD cDNAs were obtained from OriGene (catalog no. SC11950) and Sino Biological (catalog no. MG50539-M), respectively. .. Mature human and mouse FD cDNA was amplified by polymerase chain reaction with the respective pro- FD cDNA as a template., The various FD cDNAs were cloned into the pCAGGS vector at the Plasmid Preparation:Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D. Article Snippet: .. The various FD cDNAs were cloned into the pCAGGS vector 21 at the Article Title: FHL2 fused with biotin ligase shuttles between focal adhesions and the nucleus in a myosin II-dependent manner Article Snippet: .. Plasmids: To generate pPB-CAG-FHL2-GFP-BirA construct, FHL2-GFP cDNA (Nakazawa et al. 2016) and BirA cDNA (Guo et al. 2014) were recombined into the Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D Article Snippet: Human and mouse pro- FD cDNAs were obtained from OriGene (catalog no. SC11950) and Sino Biological (catalog no. MG50539-M), respectively. .. Mature human and mouse FD cDNA was amplified by polymerase chain reaction with the respective pro- FD cDNA as a template., The various FD cDNAs were cloned into the pCAGGS vector at the Cloning:Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D. Article Snippet: .. The various FD cDNAs were cloned into the pCAGGS vector 21 at the Article Title: Bleb-based extravasation: conserved morphodynamics, divergent calcium control Article Snippet: .. The full-length of EGFP was amplified by PCR, and subcloned into the EcoRI-PstI site of pT2A-BItight-TRE. pT2A-BItight-EGFP-TRE-Orai1 E106Q: The ORF of human Orai1 with E106Q mutation and myc tag (addgene #22754) was amplified by PCR, and subcloned into the MluI-EcoRV site of pT2A-BItight-EGFP-TRE. pT2A-BItight-mCherry-TRE: The full-length of mCherry was amplified by PCR, and subcloned into the EcoRI-BglII site of pT2A-BItight-TRE. pT2A-BItight-GCaMP6s-2AP-mCherry-TRE: The consecutive sequences of GCaMP6s-2AP-mCherry was amplified by PCR, and subcloned into the Article Title: H3.3 G34R-engineered murine glioma reproduces diffuse perineuronal infiltration of diffuse hemispheric glioma, H3 G34-mutant. Article Snippet: A plasmid containing the fully sequenced mouse H3f3a cDNA (Accession: BC106177, Clone ID: 6511787) was purchased from GE Healthcare Dharmacon Inc. Site-directed mutagenesis was performed using primers incorporating the H3f3a-G34R mutation (GGG→CGG: Gly (G)→Arg(R; Fig. 1A). .. The mutant cDNA was amplified by PCR and ligated into the Article Title: FHL2 fused with biotin ligase shuttles between focal adhesions and the nucleus in a myosin II-dependent manner Article Snippet: .. Plasmids: To generate pPB-CAG-FHL2-GFP-BirA construct, FHL2-GFP cDNA (Nakazawa et al. 2016) and BirA cDNA (Guo et al. 2014) were recombined into the Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D Article Snippet: Human and mouse pro- FD cDNAs were obtained from OriGene (catalog no. SC11950) and Sino Biological (catalog no. MG50539-M), respectively. .. Mature human and mouse FD cDNA was amplified by polymerase chain reaction with the respective pro- FD cDNA as a template., The various FD cDNAs were cloned into the pCAGGS vector at the Amplification:Article Title: Bleb-based extravasation: conserved morphodynamics, divergent calcium control Article Snippet: .. The full-length of EGFP was amplified by PCR, and subcloned into the EcoRI-PstI site of pT2A-BItight-TRE. pT2A-BItight-EGFP-TRE-Orai1 E106Q: The ORF of human Orai1 with E106Q mutation and myc tag (addgene #22754) was amplified by PCR, and subcloned into the MluI-EcoRV site of pT2A-BItight-EGFP-TRE. pT2A-BItight-mCherry-TRE: The full-length of mCherry was amplified by PCR, and subcloned into the EcoRI-BglII site of pT2A-BItight-TRE. pT2A-BItight-GCaMP6s-2AP-mCherry-TRE: The consecutive sequences of GCaMP6s-2AP-mCherry was amplified by PCR, and subcloned into the Article Title: H3.3 G34R-engineered murine glioma reproduces diffuse perineuronal infiltration of diffuse hemispheric glioma, H3 G34-mutant. Article Snippet: A plasmid containing the fully sequenced mouse H3f3a cDNA (Accession: BC106177, Clone ID: 6511787) was purchased from GE Healthcare Dharmacon Inc. Site-directed mutagenesis was performed using primers incorporating the H3f3a-G34R mutation (GGG→CGG: Gly (G)→Arg(R; Fig. 1A). .. The mutant cDNA was amplified by PCR and ligated into the Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D Article Snippet: Human and mouse pro- FD cDNAs were obtained from OriGene (catalog no. SC11950) and Sino Biological (catalog no. MG50539-M), respectively. .. Mature human and mouse FD cDNA was amplified by polymerase chain reaction with the respective pro- FD cDNA as a template., The various FD cDNAs were cloned into the pCAGGS vector at the Polymerase Chain Reaction:Article Title: Bleb-based extravasation: conserved morphodynamics, divergent calcium control Article Snippet: .. The full-length of EGFP was amplified by PCR, and subcloned into the EcoRI-PstI site of pT2A-BItight-TRE. pT2A-BItight-EGFP-TRE-Orai1 E106Q: The ORF of human Orai1 with E106Q mutation and myc tag (addgene #22754) was amplified by PCR, and subcloned into the MluI-EcoRV site of pT2A-BItight-EGFP-TRE. pT2A-BItight-mCherry-TRE: The full-length of mCherry was amplified by PCR, and subcloned into the EcoRI-BglII site of pT2A-BItight-TRE. pT2A-BItight-GCaMP6s-2AP-mCherry-TRE: The consecutive sequences of GCaMP6s-2AP-mCherry was amplified by PCR, and subcloned into the Article Title: H3.3 G34R-engineered murine glioma reproduces diffuse perineuronal infiltration of diffuse hemispheric glioma, H3 G34-mutant. Article Snippet: A plasmid containing the fully sequenced mouse H3f3a cDNA (Accession: BC106177, Clone ID: 6511787) was purchased from GE Healthcare Dharmacon Inc. Site-directed mutagenesis was performed using primers incorporating the H3f3a-G34R mutation (GGG→CGG: Gly (G)→Arg(R; Fig. 1A). .. The mutant cDNA was amplified by PCR and ligated into the Article Title: Treatment of atypical hemolytic uremic syndrome and C3 glomerulopathy in mice by hepatic expression of factor D Article Snippet: Human and mouse pro- FD cDNAs were obtained from OriGene (catalog no. SC11950) and Sino Biological (catalog no. MG50539-M), respectively. .. Mature human and mouse FD cDNA was amplified by polymerase chain reaction with the respective pro- FD cDNA as a template., The various FD cDNAs were cloned into the pCAGGS vector at the Mutagenesis:Article Title: Bleb-based extravasation: conserved morphodynamics, divergent calcium control Article Snippet: .. The full-length of EGFP was amplified by PCR, and subcloned into the EcoRI-PstI site of pT2A-BItight-TRE. pT2A-BItight-EGFP-TRE-Orai1 E106Q: The ORF of human Orai1 with E106Q mutation and myc tag (addgene #22754) was amplified by PCR, and subcloned into the MluI-EcoRV site of pT2A-BItight-EGFP-TRE. pT2A-BItight-mCherry-TRE: The full-length of mCherry was amplified by PCR, and subcloned into the EcoRI-BglII site of pT2A-BItight-TRE. pT2A-BItight-GCaMP6s-2AP-mCherry-TRE: The consecutive sequences of GCaMP6s-2AP-mCherry was amplified by PCR, and subcloned into the Article Title: H3.3 G34R-engineered murine glioma reproduces diffuse perineuronal infiltration of diffuse hemispheric glioma, H3 G34-mutant. Article Snippet: A plasmid containing the fully sequenced mouse H3f3a cDNA (Accession: BC106177, Clone ID: 6511787) was purchased from GE Healthcare Dharmacon Inc. Site-directed mutagenesis was performed using primers incorporating the H3f3a-G34R mutation (GGG→CGG: Gly (G)→Arg(R; Fig. 1A). .. The mutant cDNA was amplified by PCR and ligated into the Article Title: Transcriptional adapter ADA2 regulates yield and end-use quality through liquid-liquid phase separation in wheat. Article Snippet: .. The full‐length GCN5, along with the introduced mutation and some domain deletions, was cloned into the Construct:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 1-181 and 1030-1218 of A. thaliana SMC1 fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Article Title: FHL2 fused with biotin ligase shuttles between focal adhesions and the nucleus in a myosin II-dependent manner Article Snippet: .. Plasmids: To generate pPB-CAG-FHL2-GFP-BirA construct, FHL2-GFP cDNA (Nakazawa et al. 2016) and BirA cDNA (Guo et al. 2014) were recombined into the Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 2-169 and 1056-1217 of M. musculus SMC1β fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Synthesized:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 1-181 and 1030-1218 of A. thaliana SMC1 fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 2-169 and 1056-1217 of M. musculus SMC1β fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Binding Assay:Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with A. thaliana proteins, A. thaliana ASY3 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 1-181 and 1030-1218 of A. thaliana SMC1 fused by a GSGSASAG linker) was synthesized by IDT and cloned into the Article Title: Direct binding of chromosome axis and cohesin complexes underlies meiotic chromosome architecture in fungi and plants Article Snippet: For yeast two-hybrid assays with M. musculus proteins, M. musculus SYCP2 fragments were PCR-amplified and cloned into the NdeI site of pGADT7 (Clontech # 630442) via isothermal assembly to generate activation domain (AD) fusions. .. The SMC1(head) construct (residues 2-169 and 1056-1217 of M. musculus SMC1β fused by a GSGSASAG linker) was synthesized by IDT and cloned into the |